Technical platform

Histocytology and microscopy

Manager(s)
Icon user
Carole
Gauron
Myriam_Collin_Photo_ID
Myriam
Collin

The platform is open to advise users who wish to set up specific protocols (guidance on other PHIV platforms is also available). Any collaborative project can be carried out by contributing to the costs of consumables and equipment. Contact the platform managers.

Contacts :

carole.gauron@ird.fr
helene.adam@ird.fr
myriam.collin@ird.fr

Conventional histology enables visualization of the major families of molecules present in plant cells and tissue structures.

The laboratory offers staining protocols for in situ visualization, at the cellular and tissue level, of primary metabolites (carbohydrates, lipids, proteins) and secondary metabolites (phenolic compounds, lignin, etc.), either on fresh tissue (vibratome sections) or on resin-embedded tissue (microtome sections).
Molecular histology permits specific visualization of molecules of interest, such as proteins, hormones, messenger RNA, etc., on fixed samples, either whole or vibratome sections, or paraffin-embedded (microtome sections).
We have extensive expertise in labelling and sectioning model and tropical plants, and are equipped with two microtomes (Microm HM 355S) and a vibratome (Microm HM 650V).
The laboratory also offers several macro and microscopy tools: a Nikon SMZ 1500 binocular magnifier equipped with a fluorescent lamp for observing large samples, screening, or dissections; three Zeiss epifluorescence microscopes (AxioImager, A1, and Z2), including one inverted microscope (Axiovert + Colibri 7) for observing samples on culture dishes or multiwell plates.
This laboratory works in close collaboration with the PHIV (Plant Histocytology and Cellular Imaging Platform) platform in Montpellier.

Keywords
  • Plant and molecular histology
  • in situ visualization
  • epifluorescence microscopy
  • microtome and vibratome sectioning
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